Patent for Stem Cells & AFA

http://livelong.eu/actual.php?aid=6

Subject: Improving mobilization, circulation in the bloodstream, differentiating and targeting tissues of the stem cells by applying therapeutically effective doses of blue-green algae, or extracts of them.

Summary:
Stem cells are pluripotent cells derived from body tissues that can be divided into more specialized cells. For example, hematopoietic stem cells play a physiological role in the continuous filling of blood cells. They can differentiate into different types of blood cells, including red blood cells, platelets and leukocytes.

Stem cells can become cells of different tissues, myocytes, hepatocytes, glial cells, and others. It was found that stem cells can cross the blood-CSF barrier and differentiate into neurons. So it is possible that stem cells be used to treat Parkinson's disease, Alzheimer's disease and traumatic brain injury. It is also shown that stem cells can turn into fibroblasts or fibroblast-like cells and to produce collagen. So it is possible that stem cells can be used for the treatment of osteogenesis imperfecta, and bone fractures.

Peterson and others found also that liver cells can arise from stem cells. Thus, stem cells may be useful in treating variety of liver diseases, including (but not limited to) cirrhosis. It is also shown that stem cells derived from bone marrow can migrate to the area of heart ​​infarction and become myocardial cells. Therefore, stem cells can be used in the treatment of myocardial infarction.

As stem cells differentiate into a wide variety of cell types, they play an important role in healing and regenerative processes in different tissues and organs. Bone marrow stem cells, including stromal cells injected into the blood of the recipient can be integrated in various organs and tissues and become mature, differentiated tissue cells permanently. Therefore activation and increased circulating stem cells may improve tissue regeneration and allow the treatment of various diseases.

Increased traffic of stem cells (e.g. CD34 +) can be achieved through the adoption of a therapeutically effective doses of blue-green algae, or extracts of it. Algal cells can be fresh, dried or canned. The increase in the number of circulating stem cells following administration of blue green algae in some cases it may be more than 500%.

Adoption of certain fractions of AFA algae (Origin&Consistence) can increase targeting of stem cells to various tissues of the body, as evidenced by the reduction in the number of circulating stem cells. For example, the application of AFA extract rich in polysaccharide increased targeting of CD34 + cells from the bloodstream to different parts of the body.

People receiving blue-green algae can be either healthy or suffering from illness or psychological disabilities.

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Terms Used:

Blue-green algae are photosynthetic gram-negative microorganisms belonging to section Cyanophyta, which exist in unicellular, colonial or fibrous bands. Representatives of blue-green algae species include Spirulina, Aphanizomenon and others. "Algae" (algae) is the plural of "alga". "Blue-green algae" refers to multiple cells from one species Aphanizomenon, multiple cells from one kind of Spirulina, or a mixture of multiple cells types Aphanizomenon, Spirulina and other species of algae.

Differentiation is the process by which cells become specialized to perform specific biological functions. Differentiation is a property that is wholly or partly lost by cancer cells.

Effective dose is the amount of blue-green algae, or extracts thereof, may trigger or increase the traffic and direct mobilization of stem cells. Therapeutically effective dose is sufficient to treat a disease or to relieve symptoms associated with nervous system disorders (such as Alzheimer's, Parkinson's, multiple sclerosis, attention deficit), traumatic brain or spinal injuries, hypertension, liver disease , chronic fatigue syndrome, irritable bowel syndrome, bacterial or viral infections, mood disorders (such as depression), or diseases of bone or cartilage.

Effective doses of blue-green algae are 0.01 to 1.0 grams per kilogram of body weight. The average effective dose for humans is 0.5 g to 15 g per day, or an average of 5 to 10 grams. Effective single-dose blue-green algae could be around 5 g. To be effective, it must be used with some frequency - one to three times a day for a week or more. The effective dose of blue-green algae and frequency of administration may depend on many factors such as type of seaweed used, if applicable, or whole cell extracts, the general health of the subject and its physiological characteristics - height, weight, body fat, metabolism, etc.

Targeting: the migration of stem cells from the circulation into tissues or organs. In some cases, targeting is done through tissue specific adhesion molecules and other processes leading to the attraction of stem cells. The attraction can be facilitated by cell receptor signal and attracting such substances released into the blood.

Stem cells: pluripotent cells that give offspring in many types of tissues, including (but not limited) to bone marrow stromal all hematopoietic cell lines. Typical stem cells reside in the bone marrow stromal or differentiated cell. If differentiated stem cell separates CD34 on their surface or respond to CD34 on the surface of other cells.

Traffic: the release of a cell from the tissue of origin and travel through its bloodstream. Trafficking involves movement of cells from the tissue of origin, by targeting adhesion to endothelium, through it, and final migration in a body. Specific, but not the only example of traffic movement of stem cells to specific organs. Another example is the movement of B cells or pre-B cells from bone marrow to the designated authority.

Detailed Description

The use of blue-green algae, such as (but not only) AFA (Aphanizomenon Flos Aquae) increases endogenous stem cells in the blood, where they can come into contact with many organs and tissues. Since stem cells are able to migrate to different tissues and organs and differentiate into functional cells, the use of blue-green algae supports tissue regeneration and anti-aging therapy.

AFA blue-green algae are applied for this purpose, although it may apply a mixture of several blue-green algae. When we say blue-green algae, we mean both goals algal cells (fresh, frozen, freeze-dried, dehydrated or otherwise preserved), and extracts from them as polysaccharide fraction.

Regardless of how adopting blue-green algae cause transient increases in the amount of circulating stem cells as CD34 +. The imrpovement of human stem cells can be measured by analyzing the response of stem cells to a dose of blue-green algae. Mobilization of stem cells can be enhanced within 1 to 5 hours after administration of blue-green algae. Mobilized stem cells enter the bloodstream and increase the number of stem cells circulating in the blood. Percent increase compared to normal output levels can be from 25% to 100% or more. Serve to control the estimated amount in the blood of this body without stimulation or the number of circulating stem cells in untreated or placebo-treated subjects.

In some cases the use of extract of blue green algae increases the speed of directing stem cells to tissues, manifested by a transient decrease in the number of circulating stem cells in the blood of the subject. The percentage decrease in circulating stem cells compared with normal baseline may be from 25% to 100% compared to control. This is both healthy testers and patients with immunosuppression, traumatic injuries, degenerative diseases, infections or chronic diseases such as skin, digestive, nervous, lymphatic, cardiovascular and endocrine systems. Some of the studies suffer from osteoporosis, heart attack, Parkinson's, Alzheimer's traumatic brain injury, multiple sclerosis, liver cirrhosis and other diseases.

Mobilization of stem cells after the use of AFA

Healthy volunteers were tested for CD34 + cells in peripheral blood before and 1 to 4 hours after consumption of 5 g dried AFA algae. Volunteers were instructed to limit physical and mental activity for some time before and after consumption of AFA. It was found that consumption of AFA induces strong transient increase in circulating blood stem cells, which is highest after 2-3 hours (up 400%) and lasts until 4:00. The consumption of AFA can increase the release of stem cells into the bloodstream from the bone marrow and their other stores.

Mobilized stem cells retain their phenotype in terms of adhesion and targeting
The surface of CD34 + stem cells mobilized into the bloodstream after consuming blue-green algae tested for adhesion molecules and targeting. The results show that the mobilized cells retain their phenotype for these markers.

Stem cells from bone marrow tissues remote village

Male mice were selected for bone marrow donors. Two groups of female mice previously irradiated with lethal doses just below, are injected male cells from bone marrow into the tail veins. The first group of 20 animals were fed with normal food. The second group of 20 animals were fed a normal diet plus 15% of blue-green algae.

Mice from each group are sacrificed at the following time points: 0, 1 week, 2 weeks, 3 weeks, 4 weeks and 8 weeks. At 2 and 8 weeks, 6 mice are killed by each group. At all other times, two mice from each group killed.

During the first two weeks after injection, blood is taken from the ears, tail or paw, and examined the population of platelets, erythrocytes and leukocytes. After killing females various tissues tested for cells of male mice. The whole brain is examined, including olfactory bulbs, hippocampus, cortical area and cerebellum. Bone marrow, heart muscle, muscles of the hind leg, liver, pancreas, parts of the intestine and lungs should be examined for the presence of male cells. It was found that male stem cells from bone marrow inhabiting some distant tissues.

A similar study was carried out using transgenic donors - male mice carrying the gene for green fluorescent protein (GFP) and isogenic recipients - female mice. Blood leukocytes and other cell types and tissues were examined for GFP and this is established, indicating that originate from the donor.

Increased population of stem cells in the traumatized tissues of mice

All bone marrow donors were male mice, all recipients were female mice. One group after sublethal radiation received donor cells and allowed ... Read more